Main Page | See live article | Alphabetical index In an isothermal titration calorimeter, the heat of reaction is used to follow a titration experiment. This permits determination of the end point (stoichiometry) of a reaction as well as its enthalpy. The technique is gaining in importance particularly in the field of biochemistry, because it facilitates determination of substrate binding to enzymes. This article is from Wikipedia. All text is available under the terms of the GNU Free Documentation License.