Kondo Isami (近藤 勇 Kondō Isami) (1834 - 1868) was a chief of the Shinsen-gumi, an armed special security team in Kyoto during the late shogunate period.

In 1863, he participated Roushi-gumi with Hijikata Toshizou and Okita Soushi. After the founder of Kiyokata Hachiro dismissed the company, they remained in Kyoto with Hijikata and Aizawa and formed Miburo Roushi gumi. They were put under the control of Kyoto Shugoshoku and worked as police in Kyoto. In an August 18 incident, his team was renamed Shinsen-gumi. In June of 1864, the Shinsengumi became well known for arresting extreme cells (Ikeda incident).

In January 1868, the Battle of Toba-Fushimi, he returned to Edo, fighting with the force depatched by the Imperial Court but lost the battle. After surrundering, he was caught on April 25 and his neck was displayed in Sanjo beach of Kyoto.