Overflow National Wildlife Refuge is a 12,247 acre wildlife refuge in Ashley County, Arkansas managed by the United States Fish and Wildlife Service.

Overflow National Wildlife Refuge was created in 1980. It lies 5 miles west of Wilmot, Arkansas. The western boundary of the refuge follows the countour along the Mississippi Alluvial Valley escarpment which separates the Mississippi River delta from the Gulf Coastal Plain. The refuge also manages 2,267 acres at the Oakwood Unit, also in Ashley County, to which there is no public access.

The refuge consists of 9,247 acres of bottomland hardwood, 2,620 acres of agricultural fields, and 200 acres of upland pine-hardwood.

The refuge was established to protect the habitat of various types of migratory waterfowl along the Mississippi Flyway. It also provides a habitat for other birds, such as the American Bald Eagle. Over 100,000 birds winter in the refuge.