St. Marie's City, in St. Mary's County, Maryland, is a small town near the southernmost end of the state, on the western shore of the Chesapeake Bay.

St. Marie's City was the original center of the Maryland colony, founded in 1634 with the arrival of the Ark and Dove colony ships.

Present day St. Marie's City is primarily the location of St. Mary's College, which runs a tourist center and archeological site devoted to colonial St. Marie's.