Samuel Rowley was an English 17th century dramatist.

There is no evidence that he was William Rowley's brother, as has been said, but he is known to have been employed by Philip Henslowe as a reader of plays. He wrote some scriptural plays which are now lost, with William Borne (or Bird, or Boyle) and Edward Juby. His only extant works are: When you see me, You know me. Or the famous Chronicle Historie of King Henry the Eight, with the birth and vertuous life of Edward Prince of Wales (1605), of interest because of its possible connexion with the Shakespearean play of Henry VIII, and The Noble Souldier. Or, A Contract Broken. justly reveng'd (1634), which was entered in the Stationers' Register as the work of Thomas Dekker, who probably wrote the major part of it.